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Image Search Results
Journal: Cell
Article Title: Western Diet Triggers NLRP3-Dependent Innate Immune Reprogramming
doi: 10.1016/j.cell.2017.12.013
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Detailed methods are provided in the online version of this paper and include the following: table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-mouse CD45 (clone 30-F11) BioLegend Cat# 103131 anti-mouse CD11b (clone M1/70) BioLegend Cat# 101245 anti-mouse CD11c (clone N418) BioLegend Cat# 117317 anti-mouse Ly6C (clone HK1.4) BioLegend Cat# 128015 anti-mouse Ly6G (clone 1A8) BioLegend Cat# 127605 anti-mouse CD8b (clone H35-17.2) eBioscience Cat# 11-0083-85 anti-mouse CD4 (clone RM4-5) eBioscience Cat# 11-0042-85 anti-mouse Ly6G (clone 1A8) BD PharMingen Cat# 553127 anti-mouse Sca-1 (clone D7) BioLegend Cat# 108120 anti-mouse cKit (clone ACK2) eBioscience Cat# 47-1172-82 anti-mouse CD16/32 (FcyRII/III; clone2.4 G2) BD PharMingen Cat# 560540 anti-mouse CD34 (clone HM34) BioLegend Cat# 128606 anti-mouse F4/80 (clone BM8) BioLegend Cat# 123117 anti-mouse MHCII (clone M5/114.15.2) BioLegend Cat# 107605 anti-mouse CD86 (clone GL-1) BioLegend Cat# 105021 anti-mouse CD45R (B220; clone RA3-6B2) eBioscience Cat# 11-0452-85 anti-mouse CD11b (clone M1/70) eBioscience Cat# 11-0112-82 anti-mouse CD3e (clone 500A2) eBioscience Cat# 11-0033-82 anti-mouse TER-119 (clone TER-119) eBioscience Cat# 11-5921-85 anti-mouse CD2 (clone RM2-5) eBioscience Cat# 11-0021-85 anti-mouse CD19 (clone 1D3) eBioscience Cat# 11-0193-85 Chemicals, Peptides, and Recombinant Proteins E.coli derived ultrapure LPS (serotype 0111:B4) Sigma-Aldrich Cat# L-4391 Anakinra (Kineret, IL-1ra) in house order via pharmacy N/A E. coli derived ultrapure LPS (serotype K12) Invivogen Tlrl-eklps Pam3CSK4 Invivogen Tlrl-pms Resiquimod (R848) Invivogen Tlrl-r848 CpG-ODN 1826 Invitrogen Tlrl-1826 Trizol ThermoFisher Scientific Cat# 15596026 Oxidized LDL Alfa Aesar Cat# J65591 E. coli derived LPS (serotype 055:B5) Sigma-Aldrich Cat# L2880 Critical Commercial Assays Infinity Cholesterol Reagents kit ThermoFisher Scientific Cat# TR13421 Mouse Cytokine single/23-plex assay BioRad Laboratories Cat# 10014905 Mouse CRP ELISA R&D Cat# MCRP00 Mouse SAA3 ELISA Invitrogen/ThermoFisher Scientific Cat# KMA0021 Human IL-6 DuoSet ELISA kit R&D Cat#DY206 Human TNF DuoSet ELISA kit R&D Cat# DY210 ProCarta Beads (IL-10, CCL5, IL-18, IL-6, TNF, MCP-1, IL- 12p40, IL-1a, IL-1b) ProCarta Bioplex Cat#
Techniques: Recombinant, Derivative Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Sample Prep, Library Quantification, Purification, Sequencing, Gene Expression, Software, Luminex, Western Blot
Journal: Cell
Article Title: Western Diet Triggers NLRP3-Dependent Innate Immune Reprogramming
doi: 10.1016/j.cell.2017.12.013
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-mouse CD45 (clone 30-F11) BioLegend Cat# 103131 anti-mouse CD11b (clone M1/70) BioLegend Cat# 101245 anti-mouse CD11c (clone N418) BioLegend Cat# 117317 anti-mouse Ly6C (clone HK1.4) BioLegend Cat# 128015 anti-mouse Ly6G (clone 1A8) BioLegend Cat# 127605 anti-mouse CD8b (clone H35-17.2) eBioscience Cat# 11-0083-85 anti-mouse CD4 (clone RM4-5) eBioscience Cat# 11-0042-85 anti-mouse Ly6G (clone 1A8) BD PharMingen Cat# 553127 anti-mouse Sca-1 (clone D7) BioLegend Cat# 108120 anti-mouse cKit (clone ACK2) eBioscience Cat# 47-1172-82 anti-mouse CD16/32 (FcyRII/III; clone2.4 G2) BD PharMingen Cat# 560540 anti-mouse CD34 (clone HM34) BioLegend Cat# 128606 anti-mouse F4/80 (clone BM8) BioLegend Cat# 123117 anti-mouse MHCII (clone M5/114.15.2) BioLegend Cat# 107605 anti-mouse CD86 (clone GL-1) BioLegend Cat# 105021 anti-mouse CD45R (B220; clone RA3-6B2) eBioscience Cat# 11-0452-85 anti-mouse CD11b (clone M1/70) eBioscience Cat# 11-0112-82 anti-mouse CD3e (clone 500A2) eBioscience Cat# 11-0033-82 anti-mouse TER-119 (clone TER-119) eBioscience Cat# 11-5921-85 anti-mouse CD2 (clone RM2-5) eBioscience Cat# 11-0021-85 anti-mouse CD19 (clone 1D3) eBioscience Cat# 11-0193-85 Chemicals, Peptides, and Recombinant Proteins E.coli derived ultrapure LPS (serotype 0111:B4) Sigma-Aldrich Cat# L-4391 Anakinra (Kineret, IL-1ra) in house order via pharmacy N/A E. coli derived ultrapure LPS (serotype K12) Invivogen Tlrl-eklps Pam3CSK4 Invivogen Tlrl-pms Resiquimod (R848) Invivogen
Techniques: Recombinant, Derivative Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Sample Prep, Purification, Sequencing, Expressing, Software, Luminex, Western Blot
Journal: Cell
Article Title: Western Diet Triggers NLRP3-Dependent Innate Immune Reprogramming
doi: 10.1016/j.cell.2017.12.013
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Detailed methods are provided in the online version of this paper and include the following: table ft1 table-wrap mode="anchored" t5 REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-mouse CD45 (clone 30-F11) BioLegend Cat# 103131 anti-mouse CD11b (clone M1/70) BioLegend Cat# 101245 anti-mouse CD11c (clone N418) BioLegend Cat# 117317 anti-mouse Ly6C (clone HK1.4) BioLegend Cat# 128015 anti-mouse Ly6G (clone 1A8) BioLegend Cat# 127605 anti-mouse CD8b (clone H35-17.2) eBioscience Cat# 11-0083-85 anti-mouse CD4 (clone RM4-5) eBioscience Cat# 11-0042-85 anti-mouse Ly6G (clone 1A8) BD PharMingen Cat# 553127 anti-mouse Sca-1 (clone D7) BioLegend Cat# 108120 anti-mouse cKit (clone ACK2) eBioscience Cat# 47-1172-82 anti-mouse CD16/32 (FcyRII/III; clone2.4 G2) BD PharMingen Cat# 560540 anti-mouse CD34 (clone HM34) BioLegend Cat# 128606 anti-mouse F4/80 (clone BM8) BioLegend Cat# 123117 anti-mouse MHCII (clone M5/114.15.2) BioLegend Cat# 107605 anti-mouse CD86 (clone GL-1) BioLegend Cat# 105021 anti-mouse CD45R (B220; clone RA3-6B2) eBioscience Cat# 11-0452-85 anti-mouse CD11b (clone M1/70) eBioscience Cat# 11-0112-82 anti-mouse CD3e (clone 500A2) eBioscience Cat# 11-0033-82 anti-mouse TER-119 (clone TER-119) eBioscience Cat# 11-5921-85 anti-mouse CD2 (clone RM2-5) eBioscience Cat# 11-0021-85 anti-mouse CD19 (clone 1D3) eBioscience Cat# 11-0193-85 Chemicals, Peptides, and Recombinant Proteins E.coli derived ultrapure LPS (serotype 0111:B4)
Techniques: Recombinant, Derivative Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Sample Prep, Purification, Sequencing, Expressing, Software, Luminex, Western Blot